Anti-Smac (Diablo Homolog, Mitochondrial, Direct IAP-binding Protein with Low pI, Second Mitochondri

Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria (reviewed in Ren et al, 2006, Shiozaki and Shi, 2004). During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways. However, the expression and role of Smac/DIABLO in primary human tumors remains to be fully elucidated. Several alternatively spliced transcript variants that encode distinct SMAC isoforms have been described, including isoform 1 (~27kD), isoform 2 (~22kD) and isoform 3 (~21kD). Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000, Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000, Immunoprecipitation: 1:50-1:200, Immunohistochemistry: Frozen, Optimal dilutions to be determined by the researcher. Positive Control: Lymphoid, many cancer cell lines, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.