Anti-SEMA4A (Semaphorin 4A, CORD10, SEMAB, SEMB)

Sema4A (Semaphorin 4A, also Sema B) is a class IV member of the semaphorin family of proteins. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. It reportedly binds to Tim­2 and Plexin D1 and B1. In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype. In the nervous system, it induces growth cone collapse in neurons. Mature human Sema4A is an 80kD (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa33­761). It contains a 651aa extracellular region (aa33­683) that is characterized by the presence of one Sema domain (aa 36­494), a PSI region (aa496­528), and an Ig­like C2­type domain (aa573­631). The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747. There are two potential splice variants. One contains a 37aa substitution for aa293­328, while a second contains an alternative start site at Met100 coupled to a deletion of aa122­154. Sema4A is reportedly cleaved to generate both a monomeric and a dimeric soluble isoforms. Over aa 32­683, human Sema4A shares 86% aa identity with mouse Sema4A. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.