Anti-REST (Repressor Element RE-1 Silencing Transcription Factor, RE1-silencing Transcription Factor

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. , REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:250-1:1000, Immunohistochemistry (paraffin): 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is reccommended., Immunoprecipitation: 2-5ug/mg lysate, Western Blot: 1:2500-1:10,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.