Anti-Protein Phosphatase 2, Methylated (formerly 2A), Catalytic Subunit, beta Isoform (Protein Phosp

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits, many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the a subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml. , Optimal dilutions to be determined by the researcher. Positive Control: Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.