Anti-Protein Phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (PPP2R1A, MGC

Alternate Name: PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, medium tumor antigen-associated 61 KDA protein, Serine/threonine protein phosphatase 2A, 65 KDA regulatory subunit A, alpha isoform, Domain Information: , Gene Ontology Terms: RNA splicing, antigen binding, binding, ceramide metabolism, cytosol, inactivation of MAPK, induction of apoptosis, membrane, microtubule cytoskeleton, mitochondrion, negative regulation of cell growth, negative regulation of tyrosine phosphorylation of Stat3 protein, nucleus, phosphoprotein phosphatase activity, protein amino acid dephosphorylation, protein binding, protein complex assembly, protein heterodimerization activity, protein phosphatase type 2A activity, protein phosphatase type 2A complex, protein phosphatase type 2A regulator activity, regulation of DNA replication, regulation of Wnt receptor signaling pathway, regulation of cell adhesion, regulation of cell cycle, regulation of cell differentiation, regulation of growth, regulation of transcription, regulation of translation, response to organic substance, second-messenger-mediated signaling, soluble fraction, Application(s): Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 60kD band observed in Human Thymus and Lymph Node lysates (calculated MW of 65.3kD according to NP_055040.2). Recommended concentration: 0.03-0.1ug/ml. 0.03ug/ml staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See P9107-14-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.