Anti-Protein Kinase G2(PKG-2)

PKGs are cyclic GMP-dependent protein kinases (also known as cGKs or PRKGs) that are classified into two types, PKG-1 and PKG-2. PKG plays an important stimulatory role in platelet activation. All PKG family members are activated by increased levels of cellular cGMP. cGMP binds to the enzyme's regulatory domain inducing a conformational change which leads to enzyme activation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.