Anti-Protein Kinase A II alpha, Regulatory Subunit (Protein Kinase A RII alpha Subunit, Protein Kina

The cAMP-dependent protein kinase (PKA) is a critical kinase involved in numerous biological functions. The PKA holoenzyme is composed of two regulatory and two catalytic subunits designated as PKA R and PKA C respectively. PKA R subunits exist as two classes, RI and RII, each containing two isoforms, alpha and beta. On the other hand, PKA C has three identified subunit isoforms, Calpha, Cbeta, and Cgamma, have been identified. The PKA R subunits allow a range of modifying agents to influence PKA activity and localization. They are found in different levels with tissue specificity. Following the bindings between PKA R subunits and the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates many cellular functions such as metabolism, ion transport, and gene transcription. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.