Anti-PLGF (Placental Growth Factor Vascular Endothelial Growth Factor-related Protein, Placental Gro

Placental growth factor (PLGF) is a dimeric glycoprotein and member of the vascular endothelial growth factor (VEGF) family, involved in angiogenesis, and the stimulation of endothelial cell proliferation and migration. The three isoforms PLGF1, PLGF2 and PLGF3 are present in most placental tissues and act through binding to the VEGFR-1/FLT1 receptor. The involvement of PLGF in angiogenesis has made PLGF a focus area in the study of inflammatory diseases such as rheumatoid arthritis and psoriasis, and PLGF is used as a biochemical marker in cardiovascular disease, and also detected at reduced levels during preeclampsia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 2ug/ml using 100ul/well, Western Blot: 1-2ug/ml. The detection limit for recombinant human PLGF is ~20ng/lane and ~300ng/lane under non-reducing and reducing conditions, respectively. Homodimeric recombinant PLGF migrates as an ~29kD protein., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.