Anti-Plasminogen Activator Inhibitor Type I (PAI-1)

Plasminogen activator inhibitor-1 (PAI-1) is the principal inhibitor of urokinase plasminogen activator(uPA) and tissue plasminogen activator (tPA). It belongs to the group of serpins (serine protease inhibitors). Elevated levels of PAI-1 are associated with poor prognosis in many types of cancer. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: Can be used as a coating antibody for uPA:PAI-1 complex ELISA., Optimal dilutions to be determined by the researcher. Hybridoma: NCI-Ag 4/1 myeloma cells with spleen cells from Balb/c mice. Culture Medium: RPMI 1640 with 10% fetal calf serum, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.