Anti-p15INK4b (Cyclin-dependent Kinase 4 Inhibitor B, Multiple Tumor Suppressor 2, MTS-2, p14-INK4b,

p15INK4B is an ~15kD member of the p16INK4 family. Human p15INK4B is 138aa in length and contains four ankyrin repeats, a common structural sequence found in members of the INK4 family. The first 50aa of p15INK4B share 44% sequence identity with p16INK4. Following this is an 81aa region sharing 97% sequence identity, after which the two proteins diverge. Human p15INK4B shares 83% aa sequence identity with mouse and rat p15INK4B. Functionally, p15INK4B interacts strongly with CDK4 and CDK6 and is a potent inhibitor. p15INK4B can potentially induce cell cycle arrest by acting as an effector of TGF-B. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.