Anti-NOXA (PMAIP1, phorbol-12-myristate-13-acetate-induced protein 1)

Noxa is a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis activation. Noxa is a transcriptional target of p53, p73 and BH3-only proteins, and competes with, and displaces, the proapoptotic proteins BAK1, and BIM, from the antiapoptotioc protein MCL1 (Bcl-2-related protein EAT/mcl1), thereby influencing apoptosis versus cell survival. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:16,000, Western Blot: 0.03-0.1ug/ml detects ~16kD band in Human Brain (Cerebellum) lysates (calculated MW of 6.03kD according to NP_066950.1)., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.