Anti-NMDA 2b Receptor (NMDA R2b, N-methyl-D-aspartate, GRIN2B, Glutamate receptor ionotropic N-methy

N-methyl-D-aspartate (NMDA) type receptors are glutamate-activated calcium channels that play central roles in brain function and disease(1). Activation of NMDA receptors leads to elevations in synaptic calcium levels, initiating a series of calcium dependent signaling events including activation of nitric oxide synthase and stimulation of calcium/calmodulin dependent protein kinase, and MAP kinase cascades(2). Through these signal transduction pathways, NMDA receptors mediate both synaptic plasticity and learning and memory(3). NMDA receptors are composed of multiple subunits, all receptors contain an NR1 subunit and various numbers of NR2 subunits(4). NR2B is the major type 2 receptor subunit expressed in adult forebrain. NR2 is also a major component of the postsynaptic density in hippocampus and cerebral cortex(5). In addition to mediating diverse aspects of synaptic physiology, NMDA receptors also play a major role in neurodegenerative diseases. Overactivity of NMDA receptors is implicated in numerous neurodegenerative processes including stroke, Alzheimer's disease, Huntington's chorea, and amyotrophic lateral sclerosis(6). Applications: Suitable for use in Western Blotting, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blotting: 1ug/ml , Immunohistochemistry(free-floating or frozen sections): 0.1-1ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.