Anti-NFkB1 p105/p50 (Transcription Factors of the Nuclear Factor kB, NF-kB1 p105/p50 )

Anti-NFkB1 p105/p50 (Transcription Factors of the Nuclear Factor kB, NF-kB1 p105/p50 )
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
137474.100 100 µl - -

3 - 19 Werktage*

682,00 €
 
Transcription factors of the nuclear factor k B (NF-kB)/Rel family play a pivotal role in... mehr
Produktinformationen "Anti-NFkB1 p105/p50 (Transcription Factors of the Nuclear Factor kB, NF-kB1 p105/p50 )"
Transcription factors of the nuclear factor k B (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-kB1 (p105/p50), and NF-kB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by IkB inhibitory proteins (3-5). NF-kB-activating agents can induce the phosphorylation of IkB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKa (IKK1) regulate the phosphorylation and processing of NF-kB2 (p100) to produce p52, which translocates to the nucleus (9-11). Following IKK-mediated phosphorylation of p105 NF-aB at multiple sites (Ser921, 923, 927, and 932) on its C-terminal domain, SCF/b-TrCP mediated processing produces the 50kD active form p50 (12,13). Applications: Suitable for use in ChIP, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:100, ChIP: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hersteller: United States Biological
Hersteller-Nr: 137474

Eigenschaften

Anwendung: IP, WB
Antikörper-Typ: Monoclonal
Klon: 13G55(D7H5M)
Konjugat: No
Wirt: Rabbit
Spezies-Reaktivität: human, mouse
Immunogen: Synthetic peptide corresponding to residues surrounding Gly415 of human NF-kB p105/p50. Species Sequence Homology: monkey
Format: Purified

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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