Anti-Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Westerm Blot: 1:1000-1:5000. Doublet band seen ~220-240kD., Flow Cytometry: 1ug/1x10e6 cells, Immnohistochemistry(FFPE): 1:100-1:200, Immunofluorescence (IC):: 1:50-1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.