Anti-Nebulin (NEB, DKFZp686C1456, FLJ39568, FLJ39584, NEB177D, NEM2)

Nebulin is a high molecular weight protein (500-800kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:400 shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22um from the z-line in electron micrographs., Western Blot: 1:400 detects a band at ~500kD, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.