Anti-mTOR, clone RM274 (recombinant antibody)

Anti-mTOR, clone RM274 (recombinant antibody)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
NSJ-R20291-0.1ML 100 µl - -

3 - 10 Werktage*

737,00 €
 
Antibody in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide. This recombinant mTOR antibody... mehr
Produktinformationen "Anti-mTOR, clone RM274 (recombinant antibody)"
Antibody in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide. This recombinant mTOR antibody reacts to human serine/threonine-protein kinase mTOR. It may also react to mouse or rat mTOR, as predicted by immunogen homology. Protein function: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1- mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1- pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser- 758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422'. Regulates osteoclastogensis by adjusting the expression of CEBPB isoforms. [The UniProt Consortium]
Schlagworte: Anti-MTOR, Anti-FRAP, Anti-mTOR, EC=2.7.11.1, Anti-Rapamycin target protein 1, Anti-Rapamycin and FKBP12 target 1, Anti-Mammalian target of rapamycin, Anti-Mechanistic target of rapamycin, Anti-Serine/threonine-protein kinase mTOR, Recombinant mTOR Antibo
Hersteller: NSJ Bioreagents
Hersteller-Nr: R20291

Eigenschaften

Anwendung: WB, IHC
Antikörper-Typ: Monoclonal
Klon: RM274
Konjugat: No
Wirt: Rabbit
Spezies-Reaktivität: human
Immunogen: A peptide corresponding to the human serine/threonine-protein kinase mTOR was used as the immunogen for this recombinant mTOR antibody.
Format: Purified

Handhabung & Sicherheit

Lagerung: -20°C
Versand: -20°C (International: -20°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
Hier kriegen Sie ein Zertifikat
oder , um Analysenzertifikate anzufordern.
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "Anti-mTOR, clone RM274 (recombinant antibody)"
Bewertung schreiben
oder , um eine Produktbewertung abzugeben.
Zuletzt angesehen