Anti-MAP4K1 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 1, MAPK/ERK Kinase Kinase Kinase

HPK1, a KHS protein kinase, is an upstream activator of the stress-activated protein kinase (SAPK) signaling pathway. The protein has a structure similar to other GCK (Group I) kinases and contains four consensus SH3 binding motifs, which have been shown to interact with the SH3 motifs of the MAP kinase kinase kinases MLK3 and MEKK1 and several adapter proteins that couple to tyrosine kinases, including BASH, Blnk, Clnk, Crk, CrkL and Grb2. Additionally, HPK1 has been shown to be phosphorylated and activated by the tyrosine kinase c-Abl in response to genotoxic agents. At least two mRNA transcripts have been reported. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000, Immunohistochemistry (Formalin fixed paraffin embedded): 1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.