Anti-Jumonji Domain Containing 2C (JMJD2C, JMJD2, Gene Amplified in Squamous Cell Carcinoma 1, GASC1

Jumonji Domain Containing 2C is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma. Applications: Suitable for use in Western Blot, Chromatin Immunoprecipitation and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000-1:10,000, Immunoprecipitation: 1:2-5ug/mg lysate, Chromatin Immunoprecipitation: 1:10-1:500, Optimal dilutions to be determined by the researcher. Positive Control: HeLa whole cell extract, Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.