Anti-Interleukin 6 (IL-6) (FITC)

Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes. It has diverse biological functions. It stimulated B cell differentiation and antibody production. It synergizes with IL-3 in megakaryocyte development and platelet production. It induces expression of hepatic acute phase proteins. It regulated bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130. Applications: Suitable for use in Flow Cytometry, FLISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1-10ul stains 10e6 human peripheral blood mononuclear cells stimulated with LPS. Successfully detects intracellular IL-6 levels., FLISA: detection, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC comjugates are sensitive to light., , Note: Applications are based on unconjugated antibody.