Anti-Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta Inducing Factor,

Interleukin 1 (IL-1) activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL-1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL-1 alpha) and interleukin1 beta (IL-1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilution: Neutralization: Measured by its ability to neutralize IL­1beta/IL­1F2­induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.001­0.003ug/ml in the presence of 50pg/ml recombinant Human IL-1beta/IL-1F2 and 1.25ug/ml concanavalin A., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.