Anti-Hemoglobin

Hemoglobin, an iron-containing metalloprotein present in red blood cells and involved in oxygen transport. Hemoglobin is a tetramer consisting of two alpha chains and two beta chains, with each chain containing a heme group. Each heme group contains an iron atom which is responsible for the binding of oxygen. Mutations in the genes for the hemoglobin protein result in a group of hereditary diseases in humans termed hemoglobinopathies, and include sickle-cell disease and thalassemias. Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66kD with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia, levels in the feces are indicative of various clinical conditions. , Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.