Anti-HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Potassium Channel 4)

Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:200 using ECL on rat brain membranes., Immunohistochemistry: mouse brain sections, Note: Dilutions should be made using a carrier protein such as BSA (1-3%). , Optimal dilutions to be determined by the researcher. Control Antigen Included: H1827-28A: HCN4, Control Antigen , Reconstitute with 100ul sterile PBS. For Positive Control in Western Blot, use 20ng of protein per Minigel lane. For negative control preincubate 3ug of fusion protein with 1ug antibody for 1hour at RT. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.