Anti-GAPDH (C-Term) Rabbit Monoclonal Antibody (RM114)

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the conversion of glyceraldehyde-3-phosphate (Cay-17865) to 1,3-bisphosphoglycerate during glycolysis and is involved in numerous additional cellular processes, including intracellular trafficking, receptor-mediated signaling, apoptosis, DNA repair, and the oxidative stress response. It exists as a tetramer and is composed of an N-terminal domain, which contains binding sites for NAD+, phosphatidylserine, RNA, and glutathione, and a C-terminal catalytic domain. GAPDH is widely expressed and primarily localizes to the cytosol, where it has roles in glycolysis and intracellular trafficking. It also localizes to the nucleus, mediating DNA integrity, gene transcription, and apoptosis, as well as to cellular membranes, where it has roles in membrane fusion and iron transport. GAPDH expression is increased by insulin, hypoxia-inducible factor-1 (HIF-1), p53, and nitric oxide (NO) and decreased by acetylated histones. Aberrant mRNA and protein levels of GAPDH have been found in tumor biopsies from patients with a variety of cancers, including lung, renal cell, colorectal, or breast cancer. Cayman's GAPDH (C-Term) Rabbit Monoclonal Antibody (RM114) can be used for immunocytochemistry (ICC), immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and Western blot (WB) applications. The antibody recognizes the C-terminal region of GAPDH at approximately 36 kDa.Synonyms: G3PD Protein, Human, GAPD Protein, Human, Glyceraldehyde-3-phosphate dehydrogenase, HEL-S-162eP Protein, Peptidyl-cysteine S-nitrosylase. Immunogen: Peptide from the C-terminal region of GAPDH. Clone Designation: RM114. MW: ~36 kDa. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ICC, IP, ChIP, WB. Cross Reactivity: (+) GAPDH.