Anti-GABAA Receptor alpha3 Subunit (N-Term)

GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS. They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: alpha1-6, beta1-4, gamma1-3, delta, epsilon, pi, theta, and rho1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by alpha1beta2gamma2 subunit-containing GABAA receptors, the major isoform found in the brain. The alpha subunit of GABAA receptors interfaces with a beta subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the gamma subunit. GABAA alpha3 subunit expression is decreased in neocortical tissue samples surgically isolated from patients with medically refractory temporal lobe epilepsy. Knockdown of Gabra3, which encodes the alpha3 subunit isoform, prevents stress-induced colonic inflammation in mice. Cayman's GABAA Receptor alpha3 Subunit (N-Term) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor alpha3 subunit at approximately 51 kDa from mouse and rat samples.Synonyms: gamma-Aminobutyric Acid Receptor Subunit alpha3, GABAA Receptor Subunit alpha3, GABRA3. Immunogen: Synthetic peptide from the N-terminal region of the alpha3 subunit of the rat GABAA receptor. MW: ~51 kDa. Formulation: (Request formulation change), 100 µl of affinity-purified polyclonal antibody. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: IHC, WB.