Anti-GABAA Receptor alpha1 Subunit

GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS. They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: alpha1-6, beta1-4, gamma1-3, delta, epsilon, pi, theta, and rho1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by alpha1beta2gamma2 subunit-containing GABAA receptors, the major isoform found in the brain. The alpha subunit of GABAA receptors interfaces with a beta subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the gamma subunit. Mutations in GABRA1, which encodes the alpha1 subunit isoform, are found in patients with autosomal dominant juvenile myoclonic epilepsy (JME), infantile spasms, and childhood absence epilepsy (CAE). GABRA1 expression is upregulated in ipsilateral, but not contralateral, perilesional tissue in a rat model of cortical ischemia-reperfusion injury. Cayman's GABAA Receptor alpha1 Subunit Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor alpha1 subunit at approximately 51 kDa from mouse and rat samples.Synonyms: gamma-Aminobutyric Acid Receptor Subunit alpha1, GABAA Receptor Subunit alpha1, GABRA1. Immunogen: Fusion protein from the cytoplasmic loop of the alpha1 subunit of the rat GABAA receptor. MW: ~51 kDa. Formulation: (Request formulation change), 100 µl of affinity-purified polyclonal antibody. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: IHC, WB.