Anti-Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate

Folate receptor 1 (FOLR1, also folate receptor alpha, folate binding protein 1, and MOv18) is a 37-42kD member of the folate receptor family. FOLR1 is expressed in reticulocytes and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The human FOLR1 preproprecursor is 257aa in length. It codes for a GPI-linked glycoprotein that is 210 aa in size (aa25-234). There are two forms of FOLR1. One is GPI-linked, while the other is a soluble MMP cleavage product (aa25-226). There is one potential alternate splice form that shows a five aa substitution for aa 121-205. Over aa 25-233, human FOLR1 shares 83% aa identity with mouse FOLR1. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 MCR-7 cells in a total volume <200ul. Wash cells in Flow Cytometry Staining Buffer (F4568-10) or other BSA-supplemented saline buffer., Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well., Western Blot: 1ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.