Anti-Fibroblast Growth Factor Receptor 3 (FGFR3)

Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot (Indirect): 1:1000, 293T cells were transfected with FGFR3 plasmide. NBT/BCIP was the substrate. Band at ~110kD., Immunoprecipitation: 0.5-1ug antibody using a whole cell lysate of transfected 293T cells expressing recombinant human FGFR3., Immunohistochemistry (Formalin-fixed paraffin): 1:250 using Tyrpsin-digested sections. Stains human placenta and umbilical cord. Staining is observed in amniotic epithelium, tubules and squamous epithelium., Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysates of transfected 293T (embryonic kidney) cells expressing recombinant human FGFR3. Recommended Secondary Antibodies (WB, IP): I1904-40B: IgG, H&L, X-Adsorbed (AP) Pab Gt x Rb, Recommended Secondary Antibodies (IHC): I1904-40N: IgG, Fc, X-Adsorbed (Biotin) Pab Gt x Rb, I1904-40B: IgG, H&L, X-Adsorbed (AP) Pab Gt x Rb, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.