Anti-Factor VIIIc, clone 2Q2133

Factor VIII is also known as Coagulant component, Antihemophilic factor, AHF. Factor VIII makes up only about 1% of the ciruculating FactorVIII/vWF complex with the bulk of the complex being vWF (99% by mass). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Indirect): 1:16,000., Western Blot: Precipitate the FactorVIII/vWF out of plasma prior to detection of Factor VIII with F0016-18A. When perfoming Western Blot of plasma preparations, background, non-specific bands, caused by the serum proteins are common. More complex blocking solutions or higher salt concentrations can help remove non-specific bands. Consult reference 2 (Rotblat, et al., Biochemistry 24(16): 4294-4300 (1985)) for additional tips with regard to detecting Factor VIII via Western Blot, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.