Anti-erbB4 (c-erbB4, HER4)

erbB4/HER4 is the fourth member of the human epidermal growth factor receptor (EGFR) family that is frequently overexpressed in a variety of aggressive epithelial carcinomas. Its extracellular domain is most similar to the orphan receptor HER3, whereas its cytoplasmic kinase domain exhibits 79% and 77% identity with EGFR and HER2, respectively. HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain and cerebellum. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 detects erbB-4 on 10ug of A431 lysates., Immunohistochemistry: formalin-fixed, paraffin-embedded sections., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.