Anti-Cysteine String Protein (CSP)

Cysteine string protein (CSP) is a 34kD rat brain protein localized to the cytoplasmic surface of the synaptic vesicle, possibly via palmitoylation (1,2). CSP homologs are localized to the nervous system of Drosophila and to cholinergic vesicles of Torpedo (3,4). The amino terminus of CSP contains the J domain, a consensus sequence found on a DnaJ like protein which is a member of the heat-shock protein family (5). Since heat shock proteins serve as chaperones that participate in protein folding and protein complex formation, CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking. Evidence for a role of CSP in presynaptic neurotransmitter release has come from genetic and physiological study in which the Drosophila CSP null mutant fails to elicit synaptic potential upon high frequency stimulation (6). CSP null mutants have a lethal phenotype and for the small percentage of mutant flies that survive, they become paralyzed and die at elevated temperature (7)., , Applications:, Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot (Colorimetric): 1:2000 , Immunoprecipitation: 0.5ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.