Anti-CXCR2

CXCR2 is a member of the GPCR family (subfamily, chemokine). It is a receptor for Interleukin 8 (IL8), which is a powerful neutrophil chemotactic factor. Binding of IL8 with the receptor activates neutrophils. This response is mediated by a second messenger system in which G-protein activates phosphatidyl inositol-calcium. This receptor binds to IL8, GRO/MGSA, and NAP2 with a high affinity. Its expression has been reported to be in a wide variety of tissues. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-2ug/ml, Immunohistochemistry (Paraffin): 5-10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.