Anti-CX3CR1, EL (Fractalkine Receptor, V28, CMKBRL1)

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3 CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively, (1-4). The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1 (5). Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine (6). CX3CR1 mediates leukocyte migration and adhesion (5). CX3CR1 is expressed in a variety of human tissues and cell lines (2). Application: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: 10ug/ml/, Western Blot: 1ug/ml, , Optimal dilutions to be determined by the researcher. Positive Control: THP-1 cell lysate. Detects a band at approximately 50kD. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.