Anti-Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Ch

The collagen family is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral selfassociation in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function, such as bone strength and platelet activation. Applications: Suitable for use in ELISA, Dot and Slot Blot, Indirect Immunofluorescence and Indirect Immunohistochemistry. Not recommended on Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 1:3000, Immunofluorescence (Indirect): 1:20-1:40, Immunohistochemistry (Indirect): 1:20-1:40. Use frozen or acetone fixed specimens (paraffin not recommended), Dot/Slot Blot: 1:300, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.