Anti-CD66b (CEACAM-8, CEA-related cell adhesion molecule 8, CGM6, CEA gene family member 6, NCA-95,

CD66b is a 95-100kD GPI anchored molecule and is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:50-1:100. Use 10ul labels 5x10e5 cells or 100ul whole blood. , Immunohistochemistry (frozen): 1:25-1:100., Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1903-13A: IgG, F(ab')2, X-Adsorbed (PE) Pab: Gt xMo, I1904-19C: IgG, F(ab')2 (HRP) Pab Gt xMo, Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control, Recommended Positive Control Tissue: Bone Marrow, Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light., , Note: Applications are based on unconjugated antibody.