Anti-CD34CD34 (Endothelial Cell Marker, Cluster designation 34, Hematopoietic progenitor cell antige

Human CD34 is an unusual glycosylated membrane protein exclusively expressed on immature hematopoietic cells and on endothelial cells. CD34 may have a role in cell adhesion. CD34 has extensive utility for bone marrow transplantation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed paraffin-embedded sections., Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured KG-1 cells were washed and incubated 45 minutes on ice with 80ul of C2386-08E at a concentration of 10ug/ml., 2. Cells were washed twice and incubated with 50ul of IgG (FITC) goat anti-mouse., 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 0.7 log10 fluorescent units when compared to a Mouse IgG3 negative control at a similar concentration. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.