Anti-CCRL2 (C-C Chemokine Receptor-like 2, Chemokine Receptor CCR11, Chemokine Receptor X, Putative

CCRL2, also known as CRAM-A, CRAM-B, CKRX, and HCR, is a seven transmembrane G protein-linked receptor that shares homology with other human chemokine receptors. Two isoforms were reported and designated CRAM-A and CRAM-B that differ at their N-termini by the inclusion of an additional 12aa in CRAM-A. CCRL2 is expressed at varying levels on a variety of peripheral blood cells, including monocytes, neutrophils, and T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 cells. Validated using human PBMCs cultured with Recombinant Human IL-2., Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1.Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction., 2.After blocking, add 10ul of C2099-81D to up to 1 x 10e6 cells and incubated for 30 minutes at RT., 3.Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog# F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer., 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2a antibody. Excitation: 620-650nm , Emission: 660-670nm, Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.