Anti-Atg12 (autophagy-related 12 4931423H11Rik, A330058M13Rik, Apg12, Apg12l, APG12-like, Atg12l)

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG12 is the human homolog of a yeast protein involved in autophagy. APG12 is conjugated to APG5. The APG12-ATG5 conjugate has an apparent molecular mass of 65kD. The C-terminal glycine of APG12 is conjugated to a central lysine (lys130) of APG5. Studies showed that overexpression of APG3 facilitated formation of the APG12-APG5 conjugate, suggesting that APG3 cross-talks with the APG12 conjugation system. APG12 is ubiquitously expressed. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.