Anti-ASH2 (ASH2L1, ASH2L2, Absent, Small, Homeotic-Like 2, Trithorax protein ASH2)

The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 on Lys4 and functions as a transcriptional co-ac-tivator (1). While yeast contain only one known Set1 protein, , mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3 and MLL4, all of which methylate his-tone H3 on Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common structural subunits WDR5, RBBP5 and ASH2L (2-6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 on Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1 histone methyltransferase complexes play a critical role in leukemogenesis (6). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1000, Western Blot: 1:2000, Immunoprecipitation: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.