Anti-alpha-Tubulin K40Ac Rabbit Monoclonal Antibody (Clone RM318)

alpha-Tubulin is a cytoskeletal protein and constituent of microtubules, a cytoskeletal assembly that has roles in a variety of cellular processes, including cell motility, division, differentiation, and intracellular transport. alpha-Tubulin is highly conserved in eukaryotes and expressed in a cell- and isotype-specific manner. There are eight human alpha-tubulin isotypes that consist of an N-terminal domain, which binds GTP and is required for microtubule self-assembly, and a variable C-terminal tail, which contains interaction sites for microtubule-associated proteins (MAPs) and is subject to a variety of post-translational modifications that regulate microtubule function and stability. alpha-Tubulin can be acetylated at a variety of sites, including on the luminal side of microtubules at lysine 40 (K40Ac), by alpha-tubulin N-acetyltransferase 1 (alpha-TAT) and deacetylated primarily by histone deacetylase 6 (HDAC6). alpha-Tubulin K40Ac is a marker of stable microtubules that recruits dynein and kinesin-1 to microtubules. It is also involved in regulating microtubule architecture as well as immune and viral responses. The levels of alpha-tubulin acetylation are decreased in a variety of neurological disorders, increased in various cancers, and dysregulated in immune disorders. Cayman's alpha-Tubulin K40Ac Rabbit Monoclonal Antibody (Clone RM318) can be used for immunocytochemistry (ICC) and Western blot (WB) applications.Immunogen: Peptide corresponding to alpha-tubulin K40Ac. Clone Designation: RM318. Formulation: (Request formulation change), 100 µl of protein A-purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ICC, WB. Cross Reactivity: (+) alpha-Tubulin (K40Ac), (-) alpha-Tubulin without acetylation at K40.