Anti-ADAMTS 4 (Aggrecanase I, A Disintigrin And Metalloproteinase with Thrombospondin motifs 4)

Anti-ADAMTS 4 (Aggrecanase I, A Disintigrin And Metalloproteinase with Thrombospondin motifs 4)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
A0859-60B.100 100 µg - -

3 - 19 Werktage*

961,00 €
 
ADAMTS4 is a member of the family of secreted zinc proteases with a multi-domain structure (1-3).... mehr
Produktinformationen "Anti-ADAMTS 4 (Aggrecanase I, A Disintigrin And Metalloproteinase with Thrombospondin motifs 4)"
ADAMTS4 is a member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha-2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4-6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified recombinant human ADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5mM 1,10-phenanthroline and recombinant human TIMP-3. Applications: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml, Immunoprecipitation: 25ug/ml immunoprecipitates ADAMTS4 from conditioned cell culture medium spiked with recombinant human ADAMTS4., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Hersteller: United States Biological
Hersteller-Nr: A0859-60B

Eigenschaften

Anwendung: ELISA, IP, WB
Antikörper-Typ: Polyclonal
Konjugat: No
Wirt: Goat
Spezies-Reaktivität: human
Immunogen: CHO cell-derived rhADAMTS4
Format: Affinity Purified

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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