Anti-Acid Sensitive Ion Channel 1 (ASIC1, BNAC 2, Amiloride-sensitive brain sodium channel, ACCN2)

ASIC1 is a member of a family of Na +channels that are activated by external protons. The family includes another three members ASIC2, ASIC3 and ASIC4. The ASICs are in fact part of a larger superfamily termed epithelial Na +channels (EnaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini. There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions. The ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium. Lately, ASIC1 has been implicated in processes such as learning and memory in the central nervous system. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot (Rat brain membranes): 1:200. , Immunohistochemistry: Rat brain sections , Optimal dilutions to be determined by the researcher. Control Antigen: A0570-05A, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.