HMGB1 is a multifunctional protein with various roles in different cellular compartments. In the nucleus, HMGB1 acts as a DNA-binding protein for gene transcription, gene regulation and DNA repair.1 In the cytosol, HMGB1 induces autophagy by interacting with Beclin-1 which results in its dissociation from Bcl2 and the subsequent induction of autophagy.2 In the extracellular compartment, HMGB1 is passively released by necrotic cells and acts as a chemokine and DAMP (Damage-associated molecular pattern) to trigger inflammatory responses.3 Besides, HMGB1 is also secreted from activated dendritic cells and macrophages and functions as a cytokine. It acts as a ligand for RAGE, TLR-2 and TLR-4, thus activating the signal pathways and the following inflammation responses.4 The HMGB1 released from apoptotic cells is neutralized by reactive oxygen species (ROS)-dependent oxidation and thus promotes immunological tolerance.
HMGB1 has attracted the attention of both basic and clinical researchers as it is associated with various pathologies such as cancer, sepsis, arteriosclerosis, chronic rheumatoid arthritis and nephritis.5,6,7 HMGB1 is reported as a necrosis marker and an elevated level of HMGB1 is a good indicator of effective anti-cancer therapy. Extracellular HMGB1 gained attention as it is associated with cell proliferation, invasion and metastasis, angiogenesis and immunosuppression.
HMGB1 ELISA Kits
Arigo Biolaboratories offers the most comprehensive ELISA kits to facilitate the HMGB1 research as well as for the development of anti-cancer drugs. These ELISA kits provide three big advantages compared to ELISA kits from other manufacturers.
The kits are highly specific for HMGB1. No cross reactivity with HMGB2
Suitable for multiple samples from multiple species
Easy and Fast
Only two incubations and one wash step are required
ELISA Kit Comparison
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|Human HMGB1 ELISA Kit||X||X||X|
|Mouse HMGB1 ELISA Kit||X||X||X||X|
|HMGB1 ELISA Kit||X||X||X||X||X|
- Czura C.J., Wang H., Tracey K.J., Dual roles for HMGB1: DNA binding and cytokine, Journal of Endotoxin Research 7, 4 (2001)
- Tang D. et al., Endogenous HMGB1 regulates autophagy, JCB Article 190, 881-892 (2010).
- Cai J. et al., The Role of HMGB1 in Cardiovascular Biology: Danger Signals, Antioxidants & Redox Signaling 23, 1351-1369 (2015)
- Man Y. et al., HMGB1 Signals Through Troll-Like Receptor (TLR) 4 And TLR2, Shock 26, 174-179 (2006)
- Pisetsky D.S., Erlandsson-Harris H., Andersson U., High-mobility group box 1 (HMGB1): an Alarming Mediating the Pathogenesis of rheumatic disease, Arthritis Res Ther; 10, 209 (2008)
- Alleva L. M., Budd A. C., Clark I. A., Systemic release of high mobility group box 1 protein during severe murine influenz, J Immunol. 181, 1454–1459 (2008).
- Campana L., Bosurgi L., Rovere-Querini P., HMGB1: a two-headed signal regulating tumor progression and immunity, Curr Opin Immunol. 20, 518–523 (2008).