November 2006
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UbiQapture-Q Kit |
An efficient tool for the isolation of ubiquitinylated proteins |
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The UbiQapture(TM)-Q Kit facilitates the isolation of both mono- and poly-ubiquitinylated proteins (independent of lysine residue chain linkage) from cell extracts, tissue lysates and in vitro assay solutions through use of a high-binding affinity matrix. Captured proteins can be analyzed by Western blotting using the highly sensitive ubiquitin-conjugate specific antibody provided, using antibodies to specific proteins of interest, or eluted from the matrix for subsequent biochemical characterization.
The UbiQapture™-Q matrix supplied with the Kit has superior binding characteristics compared to other commercially available matrices and is compatible with a wide range of lysate buffers and cell/tissue samples from a variety of species.
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Suggested uses:
- Isolation and detection of ubiquitinylated protein conjugates from cell/tissue lysates.
- Pull down of ubiquitinylated proteins from in vitro assays.
- Separation of ubiquitinylated/non-ubiquitinylated forms of specific proteins of interest.
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UbiQapture-Q Kit (Cat. # UW8995)
Provides sufficient material for 25 binding assays. |
Ubiquitin-binding Matrices
Structurally distinct ubiquitin modifications, including mono-ubiquitinylation and up to seven types of polyubiquitin chains, supposes the existence of recognition factors that couple specific ubiquitin signals into appropriate downstream consequences. BIOMOL offers a panel of ubiquitin-interacting domains conjugated to agarose for the capture and characterization of ubiquitinylated proteins.
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| 19S Subunit S5a (Rpn10), Agarose Conjugate (Cat. # UW8635) |
| 19S Subunit S5a (Rpn10) UIM, Agarose Conjugate (Cat. # UW9820) |
| p62-derived UBA Domain, Agarose Conjugate (Cat. # UW9010) |
| hHR23B-derived UBA2 Domain, Agarose Conjugate (Cat. # UW9440) |
| NBR1-derived UBA domain, Agarose Conjugate (Cat. # UW9445) |
| NUB1/NUB1L UBA Domain, Agarose Conjugate (Cat. # UW9700) |
| UQ1 UBA Domain, Agarose Conjugate (Cat. # UW9830) |
| Dsk2 UBA Domain, Agarose Conjugate (Cat. # UW9835) |
| VPS9-derived CUE Domain, Agarose Conjugate (Cat. # UW9450) |
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14-3-3 Proteins
14-3-3 proteins are phosphobinding proteins known to influence a wide variety of cellular processes. While distinct functions for each of the seven human isoforms are still being defined, binding of 14-3-3 proteins generally causes a conformational change or steric hindrance of the bound partner resulting in diverse functional consequences. These include altered subcellular localization, inhibition of degradation, regulation of enzyme activity, or altered association of binding partners. Over 300 proteins from diverse signaling pathways have been identified to bind 14-3-3 proteins, including Raf family members, PKCs, IGF-1R, CDC25, BAD, class II HDACs and others. In addition, 14-3-3 sigma is directly implicated in cancer progression and may be an attractive target for new cancer therapeutics.
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| Pan 14-3-3, rabbit pAb (Cat. # SA-483) Species reactivity: human, rat, mouse, cow, sheep, chicken, plants, invertebrates and yeast. Applications: WB, IP, IHC. |
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| 14-3-3 (pS185), sheep pAb (Cat. # SA-479) Recognizes the phosphorylated forms of 14-3-3 beta and zeta, which are found endogenously only in brain tissue. Species reactivity: human, mouse, rat, cow, and sheep. Applications: WB. |
| 14-3-3 beta, rabbit pAb (Cat. # SA-474) Species reactivity: human, rat, mouse, cow, and sheep. Applications: WB, IHC, ELISA, affinity chromatography. |
Western blot of rat brain lysate using Anti-14-3-3 zeta |
| 14-3-3 epsilon (C-terminus), rabbit pAb (Cat. # SA-475) Species reactivity: human, rat, mouse, rabbit, cow, sheep and chicken. Applications: WB, IHC, ELISA, affinity chromatography. |
| 14-3-3 epsilon (N-terminus), rabbit pAb (Cat. # SA-478) Species reactivity: human, rat, mouse, rabbit, cow, sheep and chicken. Applications: WB, IHC, ELISA, affinity chromatography. |
| 14-3-3 eta, rabbit pAb (Cat. # SA-476) Species reactivity: human, rat, mouse, cow, sheep and chicken. Applications: WB, IHC. |
| 14-3-3 gamma, rabbit pAb (Cat. # SA-477) Species reactivity: human, rat, mouse, cow, sheep and chicken. Applications: WB, IHC, ELISA, affinity chromatography. |
| 14-3-3 tau/theta, rabbit pAb (Cat. # SA-481) Species reactivity: human, rat, mouse, cow, sheep and chicken. Applications: WB. |
| 14-3-3 zeta, rabbit pAb (Cat. # SA-482) Species reactivity: human, rat, mouse, cow, sheep and chicken. Applications: WB, IHC, affinity chromatography. |
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| Transient Receptor Potential Vanilloid-type (TRPV) Reagents |
Transient Receptor Potential Channels
The Transient Receptor Potential (TRP) superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.
TRPV1 has many activators among them heat, protons, vanilloids such as capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation. Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.
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New TRPV Ligands |
| Evodiamine (Cat. # CA-233) A novel structural class of agonist for rat TRPV1 (Ki= 5.95 microM). |
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AMG-9810 (Cat. # CA-232) An antagonist of TRPV1 (IC50=24.5 nM).
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TRPV Antibodies |
| TRPV1, Rabbit pAb (Cat. # SA-564) Antigen: amino acids 824-838 (C-terminus) of rat TRPV1. Species reactivity: human, mouse and rat; applications: WB, IHC. |
| TRPV1, Rabbit pAb (Cat. # SA-555) Antigen: amino acid residues 1-21 of rat TRPV1. Species reactivity: rat and mouse. Applications: WB, IHC, ICC. |
Immunolabeling of rat dorsal root ganglion using the TRPV1 antibody (Cat. # SA-555) |
| TRPV1, Rabbit pAb (Cat. # SA-556) Antigen: amino acid residues 7-21 of human TRPV1. Species reactivity: human. Applications: IF, IHC. |
TRPV4, Rabbit pAb (Cat. # SA-565) Species reactivity:
mouse and rat. Applications: WB, IHC.
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| TRPV5, Rabbit pAb (Cat. # SA-566) Species reactivity: human and rat. Applications: WB, IHC. |
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Request our Neuroscience Antibodies Product Guide
This guide details over 200 highly characterized antibodies for neuroscience research. These antibodies complement an offering of neurotransmitters, ion channel modulators and drugs available online and in our catalog.
| Includes antibodies to: |
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| Neurodegenerative Disease |
Neuronal Cell Death |
| Neurotransmitter Biosynthesis Enzymes |
Neurotransmitters |
| Ion Channels |
Neurofilament |
| Exocytosis and Vesicle Trafficking |
Neurotransmitter Receptors |
| Neurotransmitter Transporters |
Ephrin Receptors |
| Request your copy today |
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| MAPK Phosphatases |
MAPK phosphatases (MKPs) comprise a group of dual specificity phosphatases which negatively regulate the MAP Kinases, dephosphorylating both the phosphotyrosine and the phosphothreonine in the -pTXpY- activation-loop motif. MKP-1 is implicated primarily in the inactivation of the stress-responsive p38 and JNK MAP kinase. MKP-3 inactivates ERK1/2 and is expressed in the heart, brain, lung, kidney and pancreas. Transcriptional suppression of MKP-3 expression has been implicated in the development of pancreatic cancer. MKPs that consist of a kinase-binding domain and a phosphatase domain exhibit little phosphatase activity in the absence of their physiological substrates. MKP binding to a specific MAPK results in enzymatic activation of the phosphatase in a substrate-induced activation mechanism.
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MKP-1, MAP Kinase Phosphatase-1 (DUSP1, CL100) (human, recombinant) (Cat. # SE-439)
Recombinant, full-length human MKP-1. Genbank accession # NM_004417. |
MKP-1/p38 Complex (human, recombinant) (Cat. # SE-440)
1:1 complex of MKP-1 with p38α. Binding of p38alpha to MKP-1 mediates its catalytic activation. |
MKP-3 (human, recombinant) (Cat. # SE-454)
Recombinant, full-length human MKP-3. Genbank accession # NM_001946. |
MKP-3/p38 (human, recombinant) (Cat. # SE-455)
1:1 complex of MKP-3 with p38alpha. Binding of MKP-3 with p38alpha results in increased phosphatase activity. |
For related products:
dual-specificity phosphatases | tyrosine phosphatases | serine/threonine phosphatases | MAPK |
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