July 2006

  
 

Featured Product
DPPIV assay and reagents

New Products
MAPK phosphatase 1
Myelin basic proteins for inducing EAE
Salvinorin A
Dopamine biosynthesis and transporter antibodies
Ubiquitin-binding Entities

Featured Products


DPPIV Activity Assay and Reagents

 


 

Representation of DPPIV structure complexed with diprotin A

 


 Kinetics of H-Gly-Pro-pNA cleavage by DPPIV
 


DPPIV is a dipetidyl peptidase responsible for degradation of the peptide hormone GLP-1, an incretin hormone that stimulates insulin secretion and biosynthesis. DPPIV inhibitors, which lead to an increase in circulating levels of GLP-1, are being tested for the treatment of type II diabetes. The DPPIV Drug Discovery Kit is an activity assay designed to identify DPPIV inhibitors. The kit includes recombinant DPPIV, chromogenic and fluorogenic substrates, control inhibitor, assay buffer and detailed instructions.

DPPIV Drug Discovery Kit (Cat. # AK-499)
Contains reagents sufficient for 96 assays


Additional DPPIV reagents

DPPIV (human, recombinant) (Cat. # SE-434)
Comprises residues Asn29-Pro766 and represents a naturally-occurring soluble form of DPPIV

GP-AMC, Fluorogenic Substrate (Cat. # P-189)
Fluorogenic substrate for DPPIV (kcat/Km=2.6 x 105 s-1M-1)

GP-pNA, Chromogenic Substrate (Cat. # P-188)
Chromogenic substrate for DPPIV

DPPIV, rabbit pAb (Cat. # SA-451)
Recognizes latent, active, and soluble forms from human, rat and mouse. Applications: WB

P32/98 (Ile-thiazolidide) (PI-142)
A competitive transition-state substrate analog inhibitor of DPPIV (Ki=30 nM). Has been used in vivo and in tissue culture


For related products:
GLP-1 peptides & antibodies | FAP antibody | proteases | protein tyrosine phosphatases
New Products
MAPK Phosphatase-1 (MKP-1) and JSP-1 Inhibitor

MKP-1 (human, recombinant) (Cat. # SE-439)
A dual specificity phosphatase which negatively regulates the MAP Kinases, dephosphorylating both the phosphotyrosine and the phosphothreonine in the -pTXpY- activation-loop motif.

MKP-1/p38 complex (Cat. # SE-440)
1:1 stoichiometric complex of recombinant human MKP-1 with recombinant human p38alpha. Binding of p38alpha to MKP-1 mediates catalytic activation both in vivo and in vitro.


BML-260 (Cat. # PR-119)
A potent inhibitor of the dual specificity phosphatase JSP-1 (IC50=18 microM).

SpeedLead-P - Phosphatase Selectivity Screening Service

SpeedLead-PTM is a new selectivity screening service provided by BIOMOL and Innova Biosciences that provides accurate target selectivity data against a panel of highly active phosphatases.

Visit www.speedleadscreen.com for more information and to request a quotation.

For related products:
PTPs | Ser/Thr Phosphatases | Dual Specificity Phosphatases | MAP Kinases
Myelin Basic Protein (MBP)
Purified MBPs ideal for inducing experimental allergic encephalomyelitis in animals.
Native MBPs purified from central nervous system tissue according to the method of Määttä et al. Can be used to induce relapsing experimental autoimmune encephalomyelitis in animals. Suitable for T-cell stimulation experiments in autoimmunity studies as all known MBP isoforms seem to be present.

J.A. Määttä et al. Biochem. Biophys. Res. Commun. 1997 238 498

Myelin Basic Protein (human, purified) (Cat. # SE-441)
Myelin Basic Protein (mouse, purified) (Cat. # SE-310)


For related products:
 neuroscience tools | glial cell markers | markers of neurological disease | neurotransmitters
Salvinorin A, Opioid Agonist


Salvinorin A (Cat. # NO-109)
Potent naturally occuring diterpenoid kappa1-opioid selective agonist.  Displays high affinity at kappa1-opioid receptors (Ki=4.3 nM).

For related products:

opioid ligands | enkephalin antibodies | FMRF amide antibody | neurotransmitters

Dopamine Biosynthesis and Transporter Antibodies
Useful markers for dopaminergic, noradrenergic, and serotonergic neurons and for applications in depression, schizophrenia, Parkinson's disease and drug abuse research.
DOPA Decarboxylase (bovine), rabbit pAb (Cat. # SA-487)
Species reactivity: rat and bovine.  Applications:  WB
Western Blot analysis of bovine adrenal medulla lysate using the human DOPA decarboxylase (DDC) antibody (SA-505).
DOPA Decarboxylase (bovine), sheep pAb (Cat. # SA-505)
Species reactivity: human, rat, rabbit, canine, guinea pig, bovine and ovine.  Applications:  WB
DOPA Decarboxylase (human), rabbit pAb (Cat. # SA-504)
Species reactivity: human, rat, rabbit, canine, guinea pig, bovine and ovine. Applications:  WB
Dopamine Beta-Hydroxylase, N-terminus (human), sheep pAb (Cat. #  SA-507)
Species reactivity: human and primate. Applications:  WB
Dopamine Beta-Hydroxylase, C-terminus (human), sheep pAb (Cat. # SA-506)
Species reactivity: human and mouse.  Applications:  WB
Dopamine Transporter, Extracellular Loop (human), rabbit pAb (Cat. # SA-509)
Species reactivity: human, mouse and primate. Applications: WB, IHC

Dopamine Transporter, C-terminus (human) rabbit pAb (Cat. #  SA-508)
Species reactivity: human, mouse and primate. Applications: WB, IHC

For related products:

dopaminergics | dopamine antibodies | dopamine metabolism and transport inhibitors

Ubiquitin-binding Entities
Ubiquitin-binding motifs conjugated to agarose useful for isolating and identifying ubiquitinylated proteins.

Dsk2 UBA-Agarose Conjugate (Cat. # UW9835)
Efficiently captures K48-linked and K63-linked polyubiquitin chains.   


UQ1-agarose binding of K48- and K63-linked ubiquitin chains analyzed by immunoblotting with an ubiquitinylated protein reactive antibody  (PW8805).

Lane 1: Multi-ubiquitin chains (Ub2-7, K48-linked) (UW8860); Lane 2: Bound; Lane 3: Unbound; Lane 4: Multi-ubiquitin chains (Ub2-7, K63-linked) (UW9570); Lane 5: Bound; Lane 6: Unbound.

UQ1 UBA Domain, Agarose Conjugate (Cat. # UW9830)
Efficiently captures mono-ubiquitin and ubiquitin conjugates including free chains in a linkage independent manner.
VPS9-derived CUE Domain, Agarose Conjugate (Cat. # UW9450)
Vps9p is a S.cerevisiae exchange factor for the yeast Rab5 GTPase homolog Vps21p. The CUE domain. The isolated CUE domain can bind multi-ubiquitin chains and ubiquitylated substrates
Additional Ubiquitin-binding Protein Agarose Conjugates:

S5a | S5a UIM | p62 UBA  | hHR23B UBA2 | NBR1 UBA | NUB1/NUB1L UBA

 Request our new Ubiquitons & Zomes Product Guide

Contains over 250 products for ubiquitin and proteasome research, a useful guide to proteasome subunit nomenclature, and review articles by R.J. Mayer, University of Nottingham and B. Dahlmann, Institute of Biochemistry, Berlin.

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