BIOMOL Online August 2006
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| Chemical Genomics |
Drug Discovery |
Epigenetics |
Neuroscience |
Signal Transduction |
Ubiquitons & Zomes |
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The HDAC Fluorimetric Cellular Activity Assay provides the reagents and protocols needed for determining rates of intracellular deacetylase activity within cultured cells and is suitable for high-throughput cell-based deacetylase assays.
HDACs are typically found in multiprotein complexes and are tightly regulated by subcellular localization, phosphorylation, and likely by other mechanisms. The HDAC Fluorimetric Cellular Activity Assay allows the determination of deacetylate activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
The kit includes Fluor de Lys Substrate, Fluor de Lys Developer, Trichostatin A (HDAC inhibitor), Nicotinamide (Sirtuin inhibitor), Fluor de Lys Deacetylated Standard, HDAC Assay Buffer, Cell Lysis Buffer, microplates, and a detailed protocol. |
HDAC Fluorimetric Cellular Activity Assay
(Cat. # AK-503)
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Bruton's Tyrosine Kinase (Cat. # SE-444)
Full-length active BTK expressed in insect cells. Suitable for kinetic assays and drug screening.
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Terreic Acid (Cat. # EI-328)
Selective inhibitor of Bruton's tyrosine kinase (BTK). Has no effect on Lyn, Syk, PKA, casein kinase I, ERK1, ERK2 and p38 kinase. |
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PKC ε (human, recombinant) (Cat. # SE-443)
Full-length active Protein Kinase C epsilon expressed in insect cells. Suitable for kinetic assays and drug screening. |
Calmodulin Kinase II (pT286), Rabbit Polyclonal Antibody (Cat. # SA-523)
Phosphorylation of threonine 286 produces an autonomously active form of the kinase. Species reactivity: rat; immunogen is 100% conserved between human, mouse, and Xenopus; application: WB
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Calmodulin Kinase II (pT305), Rabbit Polyclonal Antibody (Cat. # SA-524)
Phosphorylation of threonine 305 inhibits the kinase. Species reactivity: rat; immunogen is 100% conserved between human, mouse, cow, chicken and Xenopus; application: WB
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Reduced prices on recombinant kinases
Akt |
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BIOMOL offers over 90 recombinant kinases - view our complete offering online! |
| Ephrin Antibodies and Eph Proteins |
Ephrin ligands and Eph receptors play important roles in neuronal pathfinding and have recently been shown to regulate arterio-venous differentiation and angiogenesis. EphrinB is a transmembrane protein, which binds the EphB receptor tyrosine kinase. The ligand-receptor interaction results in signaling in both the EphB-expressing and the EphrinB-expressing cells. Signaling by the EphrinB/EphB interaction is mediated in part by tyrosine phosphorylation of EphrinB. |
EphrinB (pY298), Rabbit Polyclonal Antibody (Cat. # SA-527)
Raised against amino acids surrounding Tyr298 of Xenopus EphrinB , which is homologous to human, mouse and rat Tyr317 and chicken Tyr305. Species reactivity: human, mouse, rat, Xenopus, and zebrafish; applications: WB
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Western Blot analysis of rat testes lysate untreated (1) or treated (2) with phosphatase and probed using Anti-EphrinB (pY298). |
EphrinB (pY317), Rabbit Polyclonal Antibody (Cat. # SA-528)
Raised against amino acids surrounding Tyr317 of chicken EphrinB, which is homologous to rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310. Species reactivity: human, mouse, rat, Xenopus, and zebrafish; applications: WB
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EphrinB (pY331), Rabbit Polyclonal Antibody (Cat. # SA-529)
Raised against amino acids surrounding Tyr331 of chicken EphrinB, which is homologous to mouse and rat Tyr343, human Tyr344 and Xenopus Tyr324. Species reactivity: human, mouse, rat, Xenopus, and zebrafish; applications: WB |
EphA2 (human, recombinant) (Cat. # SE-432)
Active cytoplasmic domain expressed in insect cells. Useful for kinetic assays and drug screening.
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EphB2 (human, recombinant) (Cat. # SE-433)
Active cytoplasmic domain expressed in insect cells. Useful for kinetic assays and drug screening.
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EphB4 (human, recombinant) (Cat. # SE-405)
Active cytoplasmic domain expressed in insect cells. Useful for kinetic assays and drug screening.
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| Ubiquiton- and Zome-related Antibodies |
ADRM1 (hRpn13), Rabbit Polyclonal Antibody (Cat. # PW9910)
ADRM1 is a previously unrecognized subunit of the 19S regulatory complex, with homology to yeast Rpn13. ADRM1 recruits Uch37, the only ubituitin C-terminal hydrolase associated with mammalian proteasomes, to the 19S regulatory complex. Species reactivity: human and mouse; application: WB
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Western blot analysis of HeLa total cell protein homogenate using Anti-Adrm1 |
RanGAP1, Rabbit Polyclonal Antibody (Cat. # PW8785)
RanGAP1 is a SUMOylation target, and is a good control substrate for SUMOylation assays. Species reactivity: human; applications: WB |
Proteasome Maturation Protein (POMP/Ump1), Rabbit Polyclonal Antibody (Cat. # PW9715)
POMP is ubiquitous protein involved in proteasome biogenesis.
Species reactivity: rat, monkey and human; applications: WB, IP, IHC |
Subunit beta7, Mouse Monoclonal Antibody (clone MCP219) (Cat. # PW9395)
Species reactivity: human, mouse and rabbit 20S proteasome subunit β7; applications: WB |
FAT10 (protein derived), Rabbit Polyclonal Antibody (Cat. # PW9680)
FAT10 and FAT10 conjugates are rapidly degraded by the proteasome. Species reactivity: human and mouse; applications: WB, IP |
Subunit Rpt1 (S7), Mouse Monoclonal Antibody (clone MSS 1-92) (Cat. # PW9400)
Rpt1 is one of six ATPases found in the 19S regulatory complex of the proteasome. Species reactivity: human; applications: IP |
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www.biomol.com/ubiquitons&zomes
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