| |
May 2006 |
|
| |
|
|
NupherinTM Transfection Reagent
-
Increases transfection efficiencies 10-100 fold
-
Compatible with common transfection protocols
-
Requires no special equipment
NupherinTM dramatically enhances the performance of commercially available cationic lipids to allow high-efficiency lipofection (10-40%) of neuronal, ganglion and other “difficult to transfect” cells. Efficient lipofection is limited by the ability of transfected DNA to enter the nucleus – a particular obstacle in nondividing or slow dividing cells like neurons. Nupherin, a peptide based reagent that combines a non-classical nuclear localization sequence (M9) with a nucleotide binding domain, helps to transport DNA into the nucleus. Nupherin is simple to use - just incubate Nupherin with your DNA for 15 minutes prior to lipofection. |
| Nupherin increases transfection efficiency in NTera2 cells 40-fold. Magnification 20x |
Start increasing your transfection efficiencies today:
Nupherin (Cat. # SE-225) |
| New Products |
| Neuroscience |
Gabapentin (Cat. # CA-234)
A GABA analog which does not act at GABA receptors but inhibits L-type calcium channels via binding to the alpha2-delta subunit. Ligands acting at the alpha2-delta subunit lead to anticonvulsant, anxiolytic and neuropathic pain relieving actions. |
|
Gabapentin-lactam (Cat. # KC-160)
Gabapentin-lactam but not gabapentin reduces protein aggregates and improves motor performance in a transgenic mouse model of Huntington’s disease. |
|
Ethyl Ferulate (Cat. # FR-116)
Induces heme oxygenase-1 and protects rat neurons against oxidative stress. |
|
Maprotiline•HCl (Cat. # AR-111)
Selective norepinephrine reuptake inhibitor and clinically useful antidepressant. Blocks apoptosis of medium spiny neurons in a mouse model of Huntington's disease. |
|
|
| Proteasome |
|
|
20S Immunoproteasome (Cat. # PW9645)
Upon stimulation with IFN-gamma, the three active beta subunits, beta1 (Y), beta2 (Z) and beta5 (X) are exchanged to their immunocounterparts, namely beta1i (LMP-2), beta2i (MECL-1) and beta5i (LMP-7), resulting in altered substrate specificity. This highly purified material has been isolated from human spleen and has been characterized by both SDS-PAGE and by fluorogenic assay using peptide substrates. |
Ac-Arg-Leu-Arg-AMC (Cat. # AW9785)
Useful for assaying the trypsin-like activity of purified proteasomes. |
| NEDD8 |
UbcH12 (NEDD8 E2 conjugating enzyme) (Cat. # UW9145)
Active, full-length UbcH12, including an N-terminal His6-tag, produced in E. coli. |
NUB1/NUB1L, Rabbit Polyclonal Antibody (Cat. # PW9685)
NUB1 (NEDD8 Ultimate Buster-1) is a negative regulator of the NEDD8 conjugation system and recruits NEDD8 and its conjugates to the proteasome for degradation. Useful for NEDD8 expression studies. Species reactivity: human; Application: WB. |
NEDP1, Sheep Polyclonal Antibody (Cat. # PW9775)
NEDP1 is a human NEDD8-specific protease. Species reactivity: human; Application: WB. |
| Ubiquitin |
USP15, Rabbit Polyclonal Antibody (Cat. # PW9795)
Ubiquitin specific protease, associated with the COP9 Signalosome. Species reactivity: human and mouse. Applications: WB. |
hHR23B-derived UBA2 Domain, Agarose Conjugate (Cat. # UW9440)
Useful for immunoprecipitation and affinity purification of multi-ubiquitinylated proteins and mixtures of ubiquitin-conjugates. |
Ub+1, Rabbit Polyclonal Antibody (Cat. # PW9780)
Ub+1 is a novel form of ubiquitin produced by molecular misreading and found at elevated levels in the brains of Alzheimer's disease patients. Species reactivity: human. Applications: WB. |
|
|
Request our new Ubiquitons & Zomes Product Guide 
Contains over 250 products for ubiquitin and proteasome research, a useful guide to proteasome subunit nomenclature and review articles by R.J. Mayer, University of Nottingham and B. Dahlmann, Institute of Biochemistry, Berlin. | |
JAK2 (Cat. # SE-435)
Active, human JAK2 expressed in insect cells: amino acids 808 to C-terminus. GenBank Accession number: NM_004972. |
PI 3-Kinase (Cat. # SE-436)
Complex of human, full length p110alpha and p85alpha subunits of phosphatidylinositol 3 kinase, co-expressed in insect cells. GenBank Accession number: U79143. |
HET-0016 (Cat. # EI-350)
A potent and selective inhibitor of CYP4A and 20-HETE biosynthesis. Inhibits angiogenesis and endothelial cell sprouting in various models. |
|
Dimethyloxaloylglycine (Cat. # EI-347)
A prolyl-4-hydroxylase inhibitor that upregulates HIF activity. DMOG also inhibits FIH (Factor Inhibiting HIF), an asparaginyl hydroxylase, which enhances the HIF response. |
|
EphA2 (Cat. # SE-432)
Active, human EphA2 expressed in insect cells: amino acid 596 to C-terminus. GenBank Accession number: NM_004431. |
EphB2 (Cat. # SE-433)
Active EphB2 expressed in insect cells: amino acids 569 to C-terminus. GenBank Accession number NM_004442. |
TIE2 (Cat. # SE-437)
Active TIE2 expressed in insect cells; amino acids 771 to C-terminus. GenBank Accession number: NM_000459. |
|
|
|
For more angiogenesis products,
request our Angiogenesis product guide
| VEGF |
Angiogenesis Inhibitors |
Akt |
| HIF-1alpha |
Sphingosine-1-phosphate |
PI 3-kinase |
| MMPs |
Cytoskeleton |
Nitric Oxide | |
|
|
| |
|
|
|